Journal: Antibiotics

Article Title: Effect of Antibiotics on Clinical and Laboratory Outcomes After Mandibular Third Molar Surgery: A Double-Blind Randomized Clinical Trial

doi: 10.3390/antibiotics14020195

Figure Lengend Snippet: Pain, salivary PGE2 concentration, facial swelling, and MMO changes in the two groups at different time points.

Article Snippet: The concentration of PGE2 in saliva samples was measured using a PGE2 ELISA kit (Human PGE2 ELISA kit, EH4233, FineTest ® , Wuhan Fine Biotech Co., Wuhan, China) employing the double-antibody sandwich technique.

Techniques: Concentration Assay

Journal: Antibiotics

Article Title: Effect of Antibiotics on Clinical and Laboratory Outcomes After Mandibular Third Molar Surgery: A Double-Blind Randomized Clinical Trial

doi: 10.3390/antibiotics14020195

Figure Lengend Snippet: Mean Salivary PGE2 concentrations in the two groups at different time points.

Article Snippet: The concentration of PGE2 in saliva samples was measured using a PGE2 ELISA kit (Human PGE2 ELISA kit, EH4233, FineTest ® , Wuhan Fine Biotech Co., Wuhan, China) employing the double-antibody sandwich technique.

Techniques:

Journal: Antibiotics

Article Title: Effect of Antibiotics on Clinical and Laboratory Outcomes After Mandibular Third Molar Surgery: A Double-Blind Randomized Clinical Trial

doi: 10.3390/antibiotics14020195

Figure Lengend Snippet: Correlation assessment of salivary PGE2 concentration and clinical outcomes with other variables and together.

Article Snippet: The concentration of PGE2 in saliva samples was measured using a PGE2 ELISA kit (Human PGE2 ELISA kit, EH4233, FineTest ® , Wuhan Fine Biotech Co., Wuhan, China) employing the double-antibody sandwich technique.

Techniques: Concentration Assay

Journal: Blood Advances

Article Title: Plasmacytoid dendritic cells respond to Epstein-Barr virus infection with a distinct type I interferon subtype profile

doi: 10.1182/bloodadvances.2018025536

Figure Lengend Snippet: EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked immunosorbent assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.

Article Snippet: IFN-α Pan and IL-12p40 enzyme-linked immunosorbent assay kits were purchased from Mabtech AB, and assays were performed according to the manufacturer’s recommendations.

Techniques: Infection, Enzyme-linked Immunosorbent Assay

Journal: Blood Advances

Article Title: Plasmacytoid dendritic cells respond to Epstein-Barr virus infection with a distinct type I interferon subtype profile

doi: 10.1182/bloodadvances.2018025536

Figure Lengend Snippet: EBV infection elicits type I IFN production in huNSG mice. huNSG mice were infected with 5 × 105 RGU EBV, and blood samples were analyzed 18 hours postinfection. IFN-α (A) or IL-12p40 (B) production was measured in the serum by enzyme-linked immunosorbent assay (PBS, n = 11; EBV, n = 12). (C) IFN-α levels were compared in the serum of huNSG mice that were PBS treated (n = 6) or infected with either 5 × 105 RGU of wild-type EBV (wt, n = 6), EBNA3A-deficient EBV (3aKO, n = 6), or EBNA3C-deficient EBV (3cKO, n = 8). (D) ISG induction was measured in blood leukocyte lysates by using quantitative real-time polymerase chain reaction. n = 8 for PBS; n = 9 for EBV. Unpaired Student t tests were applied.

Article Snippet: IFN-α Pan and IL-12p40 enzyme-linked immunosorbent assay kits were purchased from Mabtech AB, and assays were performed according to the manufacturer’s recommendations.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction